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Association of A313G polymorphism of GSTP1 gene with biochemical blood parameters in patients with nonalcoholic fatty liver disease

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Background: One of the key gene involved in chronic diffuse liver diseases pathogenesis, including nonalcoholic fatty liver disease are genes encoding the synthesis of glutathione-S-transferases. The objective of the study was to investigate a possible link between A313G polymorphism of the GSTP1 gene and biochemical blood parameters in nonalcoholic fatty liver disease patients.

Methods: Gene polymorphism of GSTP1 (A313G) in 64 nonalcoholic fatty liver disease patients and 20 healthy individuals (control group) was studied. The range of investigated biochemical blood parameters included: total bilirubin and its fractions, cholesterol, triglycerides, uric acid, total protein and albumin, urea, creatinine, plasma enzyme activity.

Results: G-allele significantly more frequently by 2.47 times (OR = 2.47, CI = 1.01-6.03, p<0.05) occurred in observed patients compared with healthy individuals. Total bilirubin level in blood of patients with GG-genotype was by 19.7% (p = 0.03) and by 36.9% (p = 0.04) higher compared with AA-genotype and AGgenotype carriers respectively. Presence of G-allele was associated with increased alanine aminotransferase activity, which was significantly higher in observed patients AG- and GG-genotypes carriers compared to patients with-AA genotype at 60.7% (p = 0.03) and 51.0% (p = 0.04) respectively.

Conclusions: Frequency of G-allele of GSTP1 gene was in 2.47 times (OR = 2.47, CI = 1.01-6.03, p<0.05) higher in nonalcoholic fatty liver disease patients compared with healthy individuals in Ukrainian population. Presence of G-allele was associated with increased concentration of total bilirubin and higher activity of cytolytic syndrome in observed patients. Abbreviations: CDLD – chronic diffuse liver diseases, NAFLD – nonalcoholic fatty liver disease, GST – glutathione-S-transferase, AST – aspartate aminotransferase, ALT – alanine aminotransferase, LDH – lactate dehydrogenase, GGT – gammaglutamyl transferase, AP – alkaline phosphatase

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