Introduction. The use of stem cells in dental practice has become possible due to the phenomenal discovery in biology and biotechnology regarding the ability of stem cells, after injecting them into the recipient’s body, to enter the places of damaged tissues and restore their cellular structure. Since bone healing occurs by replacing a defect with connective tissue, our task was to transplant multipotent stem cells, which will later differentiate into the actual bone tissue.
The aim of the study was to determine the biocompatibility of mesenchymal stromal cells of adipose tissue (MMSC – AT) with osteoplastic materials.
Material and methods. The research was conducted within the Bukovinian State Medical University, Chernivtsi, Ukraine, from May 2018 to February 2019. Samples of adipose tissue were obtained from the neck area of 60 experimental animals (white Wistar line rats). We conducted an analysis of phenotypic markers multipotent mesenchymal stromal cells of adipose tissue. It was determined the degree of remineralization of these samples, which was confirmed by Alamar Blue test (AB).
Results. Analysis of the degree of mineralization of the extracellular matrix revealed that the investigated samples with the contents of multipotent mesenchymal stromal cells of adipose tissue (MMSC-AT) had osteogenic potential, which was more pronounced in the samples of MMSC-AT + platelet-rich plasma (PRP) and MMSC-AT + PRP + Kolapan. The cultured MMSC-AT tissues from experimental rats of the 2nd passage express the markers characteristic of MMSC, MMSC-AT capable of differentiation in the osteogenic direction, with the prevalence of this process in tissue samples containing Platelet-Enriched Plasma and Kolapan.
Conclusions. Consequently, the tissue equivalent of bone tissue based on MMSC-AT can be used in regenerative medicine, and also can be used in different directions of dentistry.
Keywords: mesenchymal stromal cells, adipose tissue, platelet-rich plasma, collagen.
List of abbreviations:
MMSC= multipotent mesenchymal stromal cells
MMSC-AT= multipotent mesenchymal stromal cells of adipose tissue
PRP= platelet-rich plasma
AB= Alamar Blue test
CU= conventional units
CUF= conventional units of fluorescence
Dept. of Surgery and Maxillofacial Surgery, Bukovinian State Medical University, Chernivtsi, Ukraine
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