ISSN ONLINE: 2558-815X
ISSN PRINT: 1584-9244
ISSN-L: 1584-9244

Clinical markers of immune disorders in the pathogenesis of Escherichia coli enteritis

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ABSTRACT

Background. Escherichia coli enteritis is one of the most common causes of diarrhea in developed countries and is caused usually by pathogenic strains of Escherichia coli.

Objectives. To investigate the role of reactive response of polymorphonuclear neutrophilic granulocytes (NG) of peripheral blood in the systemic inflammatory response mechanisms of acute Escherichia enterocolitis (AEC), depending on genes polymorphism of heat shock proteins (HSP) family 70-2 (HSP70-2, 1267A®G) and interleukin 10 (IL-10, C-592A).

Material and methods. The genes polymorphism was analysed by PCR based method in 95 patients with AEC and 30 healthy individuals. Clinical markers of immune disorders were evaluated after hematological indices, based upon an extended general clinical blood analysis, using verified formulas.

Results. The endogenous intoxication severity did not depend reliably on genotypes of IL-10 gene (rs1800872), however it was significantly 23.68% (р=0.043) higher in GG-genotype carriers of HSP70-2 gene (rs1061581). The reduction of cellular reactivity by 14.71-19.08% (р<0.01) did not depend on the analyzed genes genotypes. But general non-specific immune reactivity decreases 3.49-4.24 times (р<0.001) was deeper in GG-genotype carriers of HSP70-2 gene and AA-genotype carriers of IL-10 gene by 17.78% (р=0.009) and 12.37% (р=0.023) respectively. The immunologic resistance index was lower by 18.75% (р=0.024) in GG-genotype carriers than in patients with А allele.

Conclusions. Hematological indices, based upon an extended general clinical blood analysis, are indicative and reliable non-specific clinical markers of immune disorders in case of AEC.

Keywords: markers, immune disorders, genes IL-10 (rs1800872), HSP70-2 (rs1061581), E. coli enteritis.

Abbreviations: AEC – acute Escherichia coli enterocolitis, HSP – heat shock proteins, IL – interleukin, ICC – immune-competent cells, LII – leukocyte intoxication index, LPS – lipopolysaccharides, NG – neutrophilic granulocytes.

Full text sources https://doi.org/10.31688/ABMU.2019.54.1.12 How to Cite Email to Author

Corresponding author:
Larysa P. SYDORCHUK
Family Medicine Department, Bukovinian State Medical University, Ukraine
Address: Teatralna Sq. 2, Chernivtsi, Ukraine 58002
Phone +380678784456, e-mail: lsydorchuk@bsmu.edu.ua

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Sydorchuk LP, Syrota BS, Sydorchuk AR, Gerush OV, Muzyka NY, Sheremet MI, Korovenkova OM, Bohdan NS, Skrynchuk OY, Nikyfor LV, Iftoda OM, Ivashchuk SI, Kushnir OV. Clinical markers of immune disorders in the pathogenesis of Escherichia coli enteritis. Arch Balk Med Union 2019;54(1):89-96. doi.org/10.31688/ABMU.2019.54.1.12