The objective of the study was to research the dynamics of changes in oxidative and reducing systems in the blood and tissues of the pancreas, lungs and liver during the development of acute necrotizing pancreatitis.
Material and methods. The experiment involved 63 sexually-mature rabbits “Gray giant”. The degree of oxidative modification of plasma proteins was assessed by the method of I.F. Meshchyshen. The content of malonic aldehyde in the erythrocytes was determined by the method of I. D. Stalna and T.G. Gorishvili. The content of ceruloplasmin in the serum was studied by M.I. Revina method. The level of medium mass molecules was determined by the method of N.I. Gabrielyan. The oxidative modification of proteins in the tissues of the pancreas, liver and lungs was carried out using the histochemical method and original techniques.
Results. The development of acute necrotic pancreatitis in the experiment was characterized by an increase in the activity of lipids and proteins in the blood and the oxidative modification of proteins in pancreatocytes, hepatocytes and alveocytes, which is one of the leading mechanisms for their lesion. The growth of the oxidative modification of proteins in the endothelial cells of the pancreas, liver and lungs in the development of acute pancreatitis contributes to the occurrence of endothelial dysfunction, which is the leading factor in the secondary lesions of these organs.
Conclusions. Considering the important role of lipoperoxidation and oxidative modification of proteins in the mechanisms of progression of acute necrotic pancreatitis, it is pathogenically grounded to develop new effective methods of systemic and local antioxidant and anti-proteolytic effects.
Keywords: acute necrotizing pancreatitis, peroxide oxidation of lipids and proteins.
Abbreviations: MMM – medium mass molecules; МА – malonic aldehyde; POM – protein oxidative modification; ANP – acute necrotizing pancreatitis; LPO – lipid peroxidation; CP – ceruloplasmin.
Michael I. SHEREMET
Surgery Department, Bukovinian State Medical University, Ukraine
Address: Golovna str. 191/16, Chernivtsi, Ukraine, 58018
E-mail: firstname.lastname@example.org; Phone +380956064607