ISSN ONLINE: 2558-815X
ISSN PRINT: 1584-9244
ISSN-L: 1584-9244

Use of flow cytometry for the evaluation of disinfectant effectiveness

1,319 views

Flow cytometric analysis is used to evaluate various microorganisms’ susceptibility to antibacterial agents and disinfectants.
This paper describes a study where Candida albicans was used to assess the method against three disinfectants.
The disinfectants examined were: 70% – ethanol and two 70%- ethanol based commercial hand disinfectants with smoothing and cleaning additives. To detect the presence of damaged microbial cells EtBr was used. With the method, a 1 minute sample incubation time showed 36.25% +- 1.45 and 35.0% +-2,0 dead cells present as a result of commercial disinfectants-1 and -2, incubation; and 86.5% +-2.8 after the incubation with 70%- ethanol. With a 3 – minute incubation time, the corresponding results were 43.25% +-3.95; 43.5% +-2.6; 86.5% +-4.3. These were significant in multigroup comparison.
With a 5-minute incubation time, C. albicans samples showed an antiseptic activity against the chemicals under investigation of: 85.5% +-2.7; 84.5% +-2.4 and 91.3% +-3.57 respectively. Here there was no significant difference.

Full text sources
Share.

Leave A Reply