Introduction. There is a great interest in applying photobiomodulation (PBM) therapy for wound care. Exposure of PBM therapy to the wound defect at the early stages of wound healing may have a systemic effect by regulating the levels of cytokines and growth factors at different stages of the repair process.
The objective was to study the effect of PBM therapy on the expression of key regulatory molecules: interferon-gamma (IFN-γ), interleukin-10 (IL-10), platelet-derived growth factor (PDGF), and granulocyte-macrophage colony-stimulating factor (GM-CSF), which influence the development of repair processes of chronic wounds at the remodeling stage.
Materials and methods. 18 rats were randomized into intact, control, and experimental groups. Chronic wounds were simulated for rats of the control and experimental groups. Wound defects of the experimental group animals were exposed to PBM therapy. The animals were euthanized on the 21st day of the experiment. The levels of IFN-γ, IL-10, PDGF, and GM-CSF were determined in serum by enzyme-linked immunosorbent assay. Histological studies were carried out. The semi-quantitative method was used to evaluate the reepithelization stage, polymorphonuclear leucocytes, fibroblasts, new vessels, and new collagen.
Results. In our study, the use of PBM therapy increased IFN-γ and PDGF levels, as well as collagen, and decreased the number of newly formed vessels in the wound during the remodeling stage.
Conclusions. PBM therapy can be used to regulate the reparative processes of chronic wounds.
Keywords: photobiomodulation, rats, wound healing, interleukins, growth factor.
Full text sources https://doi.org/10.31688/ABMU.2024.59.4.04
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Address for correspondence:
Marina V. KUMETCHKO
Department of Surgery No. 4, Kharkiv National Medical University, 4, Nauky Ave., Kharkiv, 61022, Ukraine
E-mail: cndl@med.edu.ua
