ISSN ONLINE: 2558-815X
ISSN PRINT: 1584-9244
ISSN-L: 1584-9244

The influence of laser radiation in decontamination of bacterial plaque in patients with orthodontic treatment. A microbiological “in vitro” investigation


Background: The aim of this study was to evaluate the antimicrobial effect of laser radiation and chlorhexidine 0,2% treatment for reducing the number of pathogen oral microorganisms, especially Streptococcus mutans, adjacent to orthodontic brackets.

Materials and methods: The suspension of microorganisms was obtained from those patients who wear orthodontic brackets. The inoculated probes with this suspension were exposed to decontamination methods, represented by the Erbium laser (2760nm, 80mj, 8 Hz), 980nm GaAlAs diode laser and 0,2% clorhexidine. After the treatment, the probes were collected from around the brackets and then were incubated aerobically at 37°C. To confirm the bactericid effect of treatment, it was adopted the colony forming units method. The Microsoft Excel program, one-way ANOVA and Tukey-Kramer test were used for the statistical process.

Results: According to colony forming unit method the best bactericide effect was observed in the Erbium laser group, followed by theGaAlAs diode group comparatively with negative control group. Following one-way ANOVA analysis it can be remarked a significant difference between groups,regarding the values, resulted from the application of different experimental treatments (p<0,05).

Conclusions: The present study proves the highest efficiency of Er:YAG laser radiation on newly colony forming around orthodontic brackets decontamination.

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